Maximum projections of basal dendrites acquired in 3D with a 40x oil objective on a confocal microscope.
This transgenic plant cell is labeled with yellow fluorescent protein (YFP) fused to tubulin. This image was acquired on a wide-field fluorescent microscope. Auto-fluorescence in plants can make fluorescence imaging tricky. Methods to overcome this hurdle include spectral imaging and/or digital "un-mixing" of spurious signals.
HeLa cells transiently transfected with over-expression vectors for Sec61b-eGFP and aTubulin-mCherry.
Imaged with a stereomicroscope and a color camera. The samples were obliquely lit to provide strong contrast between image and background.
Exciting first glimpse of fresh data! Results are from forward genetic screening of bacteria labeled with GFP. The image in the center pane has some cells that show polar body localization.